Hi-Plex

Hi-Plex HPLC Columns from Agilent Technologies

Agilent's Hi-Plex HPLC column provides reliable carbohydrate analysis with simple instrument requirements. Unlike Ion Chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated polystyrene gel impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58.

Hi-Plex

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Features
  • Agilent's recommended column for accurate analysis of carbohydrates
  • Operates on a standard HPLC and does not require specialized equipment
  • Long column life and excellent batch-to-batch reproducibility
  • Wide range of counterions and column configurations to optimize resolution
  • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % ACN as a modifier
  • Available in 8 µm and 10 µm particle sizes in a range of choices for USP media types – L17, L19, L34 and L58
Specifications
Bonded Phase Temperature Range Flow Rate (mL/min) Eluent USP Designation
Hi-Plex Ca 80-90 ºC 0.6 Water L19
Hi-Plex Ca USP L19 80-90 ºC 0.3 Water L19
Hi-Plex Pb 70-90 ºC 0.6 Water L34
Hi-Plex H for carbohydrates 60-70 ºC 0.6 Water L17
Hi-Plex H for organic acids 40-60 ºC 0.6 Dilute Acid L17
Hi-Plex Ca (Duo) 80-90 ºC 0.6 Water L19
Hi-Plex K 80-90 ºC 0.6 Water  
Hi-Plex Na (Octo) 80-90 ºC 0.6 Water, Sodium Hydroxide L58
Hi-Plex Na 80-90 ºC 0.3 Water L58
Literature